Protective effect of recombinant beta-defensin-2 on acute lung injury induced by sepsis in rats.
- Author:
Zhuo SHI
1
;
Zheng-yan ZHAO
;
Qiang SHU
Author Information
- Publication Type:Journal Article
- MeSH: Acute Disease; Adenoviridae; genetics; Animals; Genetic Therapy; methods; Genetic Vectors; Lung Diseases; etiology; therapy; Male; Random Allocation; Rats; Rats, Sprague-Dawley; Recombinant Fusion Proteins; genetics; physiology; Sepsis; complications; Survival Analysis; beta-Defensins; genetics; physiology
- From: Journal of Zhejiang University. Medical sciences 2006;35(6):605-609
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the protective effect of recombinant beta-defensin-2 (BD2) on acute lung injury induced by sepsis in rats.
METHODSRecombinant adenovirus carrying an expression cassette of rat BD2 (rBD2) or control adenovirus carrying empty vector was administered intratracheally to Sprague-Dawley rats 48 h before acute lung injury, which was induced by cecal ligation and double puncture (CLP). Histological changes of the lungs and 7-day survival rate were determined.
RESULTThe alveolar damage, interstitial edema, and infiltration of neutrophils were reduced and the survival rate was increased significantly in BD2 group than those in control group (P < 0.05). The CFU of abdominal bacteria was comparable to that in the control rats (P > 0.05).
CONCLUSIONThe administration of recombinant BD2 protects lung injury and improves the survival of rats with induced sepsis.
