Effect of the disassembled recipes of dahuang zhechong pill on proliferation and apoptosis of vascular smooth muscle cells in atherosclerotic rabbits.
- Author:
Yuan-yuan JI
1
;
Jun-tian LIU
;
Jing-li LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Aorta, Thoracic; metabolism; pathology; Apoptosis; drug effects; Atherosclerosis; metabolism; pathology; Cell Proliferation; drug effects; Cells, Cultured; Drugs, Chinese Herbal; pharmacology; Immunohistochemistry; In Situ Nick-End Labeling; Male; Muscle, Smooth, Vascular; metabolism; pathology; Myocytes, Smooth Muscle; drug effects; metabolism; pathology; Proliferating Cell Nuclear Antigen; biosynthesis; Rabbits
- From: Chinese Journal of Integrated Traditional and Western Medicine 2006;26(10):913-917
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of the disassembled recipes of Dahuang Zhechong Pill (DRDZP) on proliferation and apoptosis of vascular smooth muscle cell (VSMC) of thoracic aorta in atherosclerotic rabbits.
METHODSThe atherosclerotic rabbit model was induced by high-cholesterol diet and immune injury of endothelium. Expression of proliferating cell nuclear antigen (PCNA) in VSMC was detected by SP immunohistochemical technique, and VSMC apoptosis was observed with TUNEL technique.
RESULTSCompared with the model control, PCNA expression in VSMC decreased, while apoptotic cells increased significantly in all the groups treated by DR-DZP (P < 0.01).
CONCLUSIONAll the DR-DZP could inhibit VSMC proliferation and promote its apoptosis to modulate the balance between cell proliferation and apoptosis, so as to exert antiatherosclerotic action, among which the disassembled-recipe I is the main composition to contribute to antiatherosclerotic action of Dahuang Zhechong Pill.
