Effects of emodin on proliferation cycle and apoptotic gene of human lung adenocarcinoma cell line Anip 973.
- Author:
Jia-ning LI
1
;
Fu-zhen LV
;
Jin-ling XIAO
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; metabolism; pathology; Aged; Antineoplastic Agents, Phytogenic; pharmacology; Apoptosis; drug effects; Blotting, Western; Caspase 3; metabolism; Cell Cycle; drug effects; Cell Line, Tumor; Cell Proliferation; drug effects; Dose-Response Relationship, Drug; Emodin; pharmacology; Humans; Lung Neoplasms; metabolism; pathology; Male
- From: Chinese Journal of Integrated Traditional and Western Medicine 2006;26(11):1015-1020
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the suppressive role of emodin on the growth and its effect on the proliferation cycle and apoptotic gene of human lung adenocarcinoma cell line Anip 973.
METHODSThe survival rate and the inhibitory rate of Anip 973 cell in vitro were detected by MTT colorimetric assay and cell growth curve assay at different time points under different concentration of emodin; the cell proliferation cycle and the apoptotic rate were examined with flow cytometry analysis, and Caspase-3 protein expression was measured by immunoblotting assay.
RESULTSEmodin inhibited the proliferation of Anip 973 cell at G0/G1 phase, decreased the cell ratio at S phase and activated the Caspase-3 protein. It suppressed the growth of tumor cells and raised the apoptotic rate in a concentration and time depending manner in a certain extent.
CONCLUSIONEmodin could suppress the proliferation of Anip 973 cell, and its mechanism of anticancer effect may be through activating Caspase-3, to induce apoptosis and block cell cycle.
