Preliminary study on differential expressed genes in T lymphocyte of chronic obstructive pulmonary disease patients with Fei-qi deficiency syndrome.
- Author:
Ze-Geng LI
1
;
Jia-Bing TONG
;
Bo PENG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Diagnosis, Differential; Female; Gene Expression Profiling; Humans; Male; Medicine, Chinese Traditional; Middle Aged; Oligonucleotide Array Sequence Analysis; methods; Pulmonary Disease, Chronic Obstructive; complications; genetics; immunology; Syndrome; T-Lymphocytes; metabolism; Yang Deficiency; complications; immunology
- From: Chinese Journal of Integrated Traditional and Western Medicine 2006;26(12):1082-1085
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study T lymphocyte related genes with differential expression in patients with chronic obstructive pulmonary disease (COPD) of Fei-qi deficiency (FQD) syndrome type by gene chips.
METHODSLymphocytes in peripheral blood were isolated by Ficoll technique from blood samples collected from COPD patients of FQD syndrome type, Fei-yin deficiency (FYD) syndrome type, and also from healthy subjects for control. They were sorted and purified by flow cytometry, and the different expressed genes were screened from them by gene chip technique.
RESULTSThere were 15 genes with high differential expression between patients of FQD type and those of FYD syndrome type, and between patients of FQD type and healthy subjects.
CONCLUSIONGene chip technique could be used for studying the gene expression profiles of TCM syndrome, and the T-lymphocyte related genes with differential expression in COPD patients with FQD were screened preliminarily.
