Effects of dahuang zhechong pill on expression and activity of matrix metalloproteinase in rats' hepatic stellate cells.
- Author:
Zhi-heng PAN
1
;
Yao XIE
;
Hong-wen HE
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Drugs, Chinese Herbal; pharmacology; Hepatocytes; cytology; metabolism; Male; Matrix Metalloproteinase 1; genetics; metabolism; Matrix Metalloproteinase 2; genetics; metabolism; Random Allocation; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Integrated Traditional and Western Medicine 2005;25(12):1100-1103
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effects of Dahuang Zhechong Pill (DZP) on the gene expression of matrix metalloproteinase-1 (MMP-1) in rats' hepatic stellate cells (HSC) and activity of matrix metalloproteinase-2 (MMP-2) that secreted into the culture base.
METHODSHSC were isolated from the liver of normal rats and incubated with DZP-contained drug serum. The expression of MMP-1 in the HSC was detected by quantitative reverse-transcription polymerase chain reaction (RT-PCR). The activity of MMP-2 was examined by zymography.
RESULTSDZP-contained drug serum could obviously promote the gene expression of MMP-1 in HSC. In the meantime, it could obviously increase the content and activity of MMP-2 synthesized by HSC (P < 0.05).
CONCLUSIONThe anti-fibrosis action of DZP was correlated to the promotion of HSC's gene expression of MMP-1 and increasing of the contents and activity of MMP-2.
