Effects of bushen huoxue recipe on proliferation, differentiation and mineralization of osteoblasts in rats.
- Author:
Hong-min ZHANG
1
;
Jun-chang CHEN
;
Yi-heng LIU
Author Information
- Publication Type:Journal Article
- MeSH: Alkaline Phosphatase; metabolism; Animals; Animals, Newborn; Calcification, Physiologic; drug effects; Calcium; metabolism; Cell Differentiation; drug effects; Cell Proliferation; drug effects; Cells, Cultured; Drugs, Chinese Herbal; pharmacology; Male; Osteoblasts; cytology; metabolism; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Integrated Traditional and Western Medicine 2005;25(12):1112-1114
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effects of bushen huoxue recipe (BHR) on cell proliferation, differentiation, maturation and mineralization in osteoblasts of rats cultured in vitro.
METHODSOsteoblasts from cranium of newborn SD rats were cultured by collagenase method. MTT, PNPP and ARS were used to observe the proliferation, activity of alkaline phosphatase (ALP) and the formation of mineral node of cultured osteoblasts effected by different concentrations of BHR at different time points (24, 48, 72 hrs after medication).
RESULTSDifferent concentrations of BHR could enhance the cell proliferation rate and ALP activity, the effects of BHR in high and moderate concentration were more significant in enhancing the cell proliferation rate (P < 0.01). And the number of mineral node of cultured osteoblasts treated by high and moderate concentration BHR was much more than that of the untreated osteoblasts (P < 0.01).
CONCLUSIONBHR could promote the proliferation, differentiation, and mineralization of the osteoblasts.
