Effect of ginkgo biloba extract and dipyridamole on transcription and translation of inducible NO synthbase in rabbits after myocardial ischemia-reperfusion injury.
- Author:
Qing-Jiang SONG
1
;
Shao-Hua WANG
;
Jie YANG
;
Jie SUN
;
Quanjian YAN
;
Miaozhang ZHU
;
Zhikun GUO
;
Zhiheng CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Dipyridamole; therapeutic use; Drugs, Chinese Herbal; therapeutic use; Female; Ginkgo biloba; Male; Myocardial Reperfusion Injury; drug therapy; enzymology; genetics; Myocardium; enzymology; Nitric Oxide Synthase Type II; biosynthesis; genetics; Phytotherapy; RNA, Messenger; biosynthesis; genetics; Rabbits; Random Allocation; Transcription, Genetic
- From: Chinese Journal of Integrated Traditional and Western Medicine 2006;26(3):240-243
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of Egb761, an extract of ginkgo biloba , and dipyridamole on inducible NO synthase (iNOS) in rabbits after myocardial ischemia-reperfusion injury.
METHODSAfter being established into ischemia-reperfusion injury model, 35 rabbits were divided randomly into 5 groups: Group A (the sham group), Group B (the model group), Group C (treated with dipyridamole 0.8 mg/kg), Group D (treated with Egb761, 40 mg/kg), and Group E (treated with Egb761 40 mg/kg combined with dipyridamole 0.8 mg/kg), all the medications were administered by intravenous injection 30 min after reperfusion. After administration, myocardial iNOS mRNA expression was detected by RT-PCR and western blot.
RESULTSMyocardial iNOS mRNA transcriptive expression in the 5 groups were A 0, B 157.11 +/- 17.73, C 202.6 +/- 21.84, D 356.13 +/- 24.18 and E 562.34 +/- 35.19 respectively, showing significant difference between the treated groups and group B (P <0.01). The translative expression of myocardial iNOS in the 5 groups were A 34.24 +/- 15.78, B 75.70 +/- 13.71, C 116.89 +/- 22.57, D 143.75 +/- 16.05 and E 195.09 +/- 22.25 respectively, showing significant difference between the treated groups and group B as well (P < 0.05, P < 0.01).
CONCLUSIONBoth Egb761 and dipyridamole could increase myocardial iNOS expression in transcriptive and translative levels in rabbits after myocardial ischemia-reperfusion injury, and the combined treatment of them shows a more significant effect.
