Biological function prediction of mir-210 in the liver of acute cold stress rat.
- Author:
Wen-Jin GUO
1
;
Shuai LIAN
1
;
Jing-Ru GUO
1
;
Jun-Fei ZHAI
1
;
Yu-Chen ZHANG
1
;
Yue LI
1
;
Li ZHEN
1
;
Hong JI
1
;
Huan-Min YANG
2
Author Information
1. College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163000, China.
2. College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163000, China. yanghuanmin@aliyun.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
Cell Cycle;
Cell Proliferation;
Cold Temperature;
Energy Metabolism;
Liver;
Male;
MicroRNAs;
Rats;
Rats, Wistar;
Stress, Physiological;
Up-Regulation
- From:
Acta Physiologica Sinica
2016;68(2):165-170
- CountryChina
- Language:Chinese
-
Abstract:
The study was aimed to observe mir-210 expression in liver tissue of acute cold stress rat and predict the function of mir-210 in cold stress. Thirty SPF Wistar male rats which were 12-week-old and weighed (340 ± 20) g were used. The rats were pre-fed in normal room temperature for one week, and then were randomly divided into acute cold stress group at (4 ± 0.1) °C and normal control group at (24 ± 0.1) °C. After the rats were treated with cold stress for 12 h, the liver tissue was extracted and the gene expression of mir-210 was assayed using qRT-PCR. The results demonstrated that the gene expression of mir-210 was significantly enhanced in acute cold stress group compared with that in normal control group (n = 3, P < 0.01). The bioinformatics analysis showed that mir-210 has over hundreds of target genes and four kinds of target genes such as E2F3, RAD52, ISCU and Ephrin-A3 are more relative with liver cold stress. ISCU regulates the cell respiratory metabolism and Ephrin-A3 is related with cell proliferation and apoptosis. On the other hand, up-regulated mir-210 affects the DNA repairing mechanism which usually leads to genetic instabilities. Our results suggest that cold stress-induced up-regulation of mir-210 in liver harmfully influences cell growth, energy metabolism and hereditary.
