Influence of the VEGF antibody targeted vascular therapy on the expression of collagen type I in hyperplastic.
- Author:
Yi-gang YUE
1
;
Chang-wen JIANG
;
Pei-ying LI
;
Si ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Animals; Antibodies, Monoclonal; therapeutic use; Cicatrix, Hypertrophic; metabolism; therapy; Collagen Type I; metabolism; Disease Models, Animal; Female; Gene Expression; Humans; Hyperplasia; metabolism; therapy; Mice; Mice, Inbred BALB C; Mice, Nude; Neovascularization, Pathologic; metabolism; RNA, Messenger; metabolism; Vascular Endothelial Growth Factor A; immunology
- From: Chinese Journal of Burns 2006;22(6):427-430
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the influence of the vascular endothelial growth factor( VEGF) antibody targeted vascular therapy on the expression of human collagen type I in hyperplastic scar of nude mice.
METHODSThe hyperplastic scar from one female burn patient with 1% TBSA deep-partial thickness burns were implanted into subcutaneous skin of scapular region of 48 nude mice. Three weeks later, the nude mice were divide into large dose (LA) , medium dose (MD) , small dose (SD) and control groups, with 12 mice in each group. The mice in LA,MD and SD groups were injected with 200 microl of 15,10, 5 microg/ml VEGF monoclonal antibody diluted in 0.01 mol/L PBS, respectively in the scar twice a week for 3 weeks, while those in C group were injected with equal amount of 0. 01 mol/L PBS. The area and volume of the scar in each group were calculated and histological changes were observed, and the expression of collagen type I mRNA and its protein in each group were determined 3 days after treatment.
RESULTSThe volume of scar in LA, MD, SD and C groups were (55.3 +/-4.1, 67.9 +/-5.7, 78.9 +/-5.5, 85.0 +7.3) mm(3), respectively. Compared with that in C group, the volume of the scar were significantly decreased in AD and MD groups ( P <0.05). A few number of vessels and fibroblasts were observed in LD, MD groups, with decreased number of collagen fibers arranged in order. Compared with that in C group ,The expression of procollagen type I mRNA and its protein in C group was obviously higher than those in LD and MD groups ( P < 0. 05) , but it was similar to those in SD group.
CONCLUSIONVEGF targeted vascular therapy is beneficial for the inhibition of the angiopoietins of hyperplastic scar, the expression of collagen , and the growth of scar.