Characteristics of Nicotinic Receptor Expressed in Human Retinoblastoma.
- Author:
Dae Ran KIM
1
;
Seung Kyu CHA
;
In Deok KONG
;
Se Hwan JUNG
;
Sang Hoon RAH
Author Information
1. Department of Physiology, Yonsei University, Wonju College of Medicine, Wonju, Korea.
- Publication Type:Original Article
- Keywords:
Calcium;
Nicotinic receptor;
Retinoblastoma cell
- MeSH:
Calcium;
Cell Death;
Cell Proliferation;
Hexamethonium;
Humans*;
Nicotine;
Receptors, Nicotinic*;
Retinoblastoma*
- From:Journal of the Korean Ophthalmological Society
2005;46(6):1060-1067
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To identify the characteristics and physiological function of the nicotinic receptor expressed in human retinoblastoma cells. METHODS: We measured possible nicotinic signaling in WERI-Rb-1 cells using the Ca2+ imaging technique and the patch clamp method. RESULTS: 1) Nicotine-induced [Ca2+]i rise arose entirely through Ca2+ influx, which was completely abolished by hexamethonium (100 micro M). 2) Nicotine also induced remarkable depolarization from -56.6 +/- 3.7 mV to -29.6 +/- 3.6 mV (n=4) under current clamp mode, but it failed to directly activate the T-type Ca2+ channel expressed in retinoblastoma cells. CONCLUSIONS: Nicotinic activation can increase the intracellular calcium level through calcium influx in the undifferentiated retinoblastoma cells, which may play important roles in cell proliferation, differentiation, and cell death.
