Study of lipopolysaccharide antagonizing effect of DPR-2 in vitro.
- Author:
Yi-Bin GUO
1
;
Hong-Wei CAO
;
Li-Ping CHEN
;
Yong-Ling LU
;
Jiang ZHENG
;
Guang-xia XIAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line; Drugs, Chinese Herbal; pharmacology; Endotoxins; antagonists & inhibitors; In Vitro Techniques; Limulus Test; Lipopolysaccharides; antagonists & inhibitors; Mice; Monocytes; drug effects; metabolism; Plant Extracts; pharmacology
- From: Chinese Journal of Burns 2007;23(2):104-107
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the lipopolysaccharide (LPS) antagonizing biological activity of densefruit pattany root-bark extract (DPR-2) in vitro.
METHODSThe effect of DPR-2 in neutralizing LPS (0.1 microg/L) was detected by kinetic turbidimetric limulus test. The effect of different concentrations of DPR-2 (0,8.0,16.0,32.0,64.0 mg/L) on binding of FITC-conjugated LPS (FITC-LPS,100.0 microg/L) to murine RAW264.7 cells was analyzed with laser scanning confocal microscopy. The expression of TNF-alpha and IL-6 mRNA in RAW264.7 cells after exposure to LPS (100.0 microg/L) were determined by real-time RT-PCR.
RESULTSDPR-2 could neutralize LPS (P < 0.05 or P < 0.01), and inhibit the binding of FITC-LPS to RAW264.7 cells in a dose-dependent manner when the concentration of DPR-2 was above 16.0 mg/L. Furthermore, DPR-2 could markedly inhibit the expression of TNF-alpha and IL-6 mRNA in LPS-stimulated murine RAW264.7 cells.
CONCLUSIONDPR-2 exhibit an anti-LPS effect in vitro, which may be related to its capacity to neutralize LPS and inhibit binding of LPS for its receptors.
