Study on the drug-resistant genes associated with beta-lactams and aminoglycosides in clinically isolated Pseudomonas aeruginosa.
- Author:
Jian-Rong FU
1
;
Qun LIU
;
Yan-Hong ZHANG
;
Jin-Wei LIU
;
Jing LIU
;
Jian LI
Author Information
- Publication Type:Journal Article
- MeSH: Aminoglycosides; pharmacology; Drug Resistance, Multiple, Bacterial; genetics; Humans; Pseudomonas aeruginosa; drug effects; genetics; isolation & purification; beta-Lactam Resistance; genetics; beta-Lactams; pharmacology
- From: Chinese Journal of Burns 2007;23(2):108-111
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate drug-resistant genes associated with beta-lactams and aminoglycosides in clinically isolated Pseudomonas aeruginosa.
METHODSTwenty strains of Pseudomonas aeruginosa were isolated from wound excretion of hospitalized burn patients. The strains resistant to 14 antibiotics were selected for detection of 16 kind of drug-resistant genes (TEM, SHV, OXA-10 cluster, PER, VEB, GES, CARB, CTX-M- I, IMP, VIM, SPM, GIM, DHA, MOX, FOX, oprD2) and 6 kind of aminoglycoside modification genes (aac(3)- I, aac(3)-II, aac(6')-I, aac(6')-II, ant (3")- I , ant(2")- I) in them by PCR.
RESULTSAmong the 20 strains resistant to beta-lactam , all of them were TEM and GES positive (100%), oprD2 gene depletion in 5 strains (25%). All other genes were negative. Among aminoglycoside resistant genes, 20 strains were aac (6') - I positive (100%), 7 were ant (2") - I positive (35%), and negative for other stains.
CONCLUSIONThere were very high existence rates of TEM, GES and aac (6')- I genes in Pseudomonas aeruginosa isolated from clinical burn patients. The fact that GES-5 gene has also been detected in Pseudomonas aeruginosa, suggesting this organism is highly drug resistant in our burn unit.
