Effects of Panax notoginseng on the transdifferentiation of fibroblasts in human hypertrophic scar in vitro.
- Author:
Heng YAO
1
;
Shi-Rong LI
;
Jian-Yi LIU
;
Zhe LI
;
Jun WU
Author Information
- Publication Type:Journal Article
- MeSH: Cell Division; drug effects; Cell Transdifferentiation; Cells, Cultured; Cicatrix, Hypertrophic; pathology; Fibroblasts; cytology; drug effects; Ginsenosides; pharmacology; Humans; Panax notoginseng; chemistry; Wound Healing
- From: Chinese Journal of Burns 2007;23(3):188-190
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effects of Panax notoginseng on the transdifferentiation of the cultured human fibroblasts from hypertrophic scar in vitro, and explore its anti-fibrosis mechanism.
METHODSThe fibroblasts from human hypertrophic scar were cultured in vitro. Different amount of Panax notoginseng was added into the medium, respectively (400 microg/ml and 800 microg/ml). A culture without addition of the drug served as control. The fibroblast-populated collagen lattice method was used to detect the gel contraction, and contraction ratio was calculated. The immunocytochemistry staining method was used to detect the expression of alpha-smooth muscle actin. The flow cytometry method was used to detect the positive rate of alpha-smooth muscle actin.
RESULTSThe contraction degree of the fibroblasts after PNS administration was ameliorated at each time-point, with contraction index lower than that of controls (P < 0.05 or P < 0.01). Scattered distribution of alpha-SMA positive granules were observed in the cytoplasma, and the positive rate of alpha-SMA expression in 400 microg/ml (31.52%) and 800 microg/ml (24.28%) PNS groups were obviously lower than that in control group (45.74%, P < 0.05). The staining intensity of positive cells in 400 microg/ml and 800 microg/ml PNS groups was also obviously lower than that in control group (P < 0.05 or P < 0.01).
CONCLUSIONPanax notoginseng can inhibit the transdifferentiation of the cultured human fibroblasts from hypertrophic scar, and it exhibits an anti-fibrosis effect on hypertrophic scar in vitro.