Influence of human C-type natriuretic peptide on vascular endothelial cell proliferation.
- Author:
Le XIAO
1
;
Yong-Ming DANG
;
De SHI
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line; Cell Proliferation; Endothelial Cells; cytology; Humans; Natriuretic Peptide, C-Type; genetics; Plasmids; RNA, Messenger; genetics; Transfection
- From: Chinese Journal of Burns 2007;23(3):191-194
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the influence of human C-type natriuretic peptide (hCNP) on proliferation of vascular endothelial cells (HUVECs).
METHODSReconstructed pcDNA3.1 (+)/hCNP was transfected into HUVECs with polyethylenimine and its plasmid expression was examined with RT-PCR, immunohistochemistry and Western blot. MTT method was used to determine the effect of expressed protein on proliferation of HUVECs. pcDNA3.1 (+)/hCNP transfection was used for control.
RESULTSThe proliferation of HUVEC 48 h after pcDNA3.1 (+)/hCNP transfection was (0.301 +/- 0.096), which was obviously higher than that with pcDNA3.1 (+) transfection (0.164 +/- 0.012). Reconstructed pcDNA3.1 (+)/hCNP might be expressed in HUVECs effectively and its protein expression was capable of promoting HUVECs proliferation markedly.
CONCLUSIONThe successive expression of reconstructed pcDNA3.1 (+)/hCNP and the promoting activity of its expressed protein on HUVECs lay the foundation potential therapeutic value of C-type natriuretic peptide.
