Anti-cervix-cancer effect of the co-immobilization of tumor necrosis factor-alpha and interferon-gamma.
- Author:
Yangqing GUAN
1
;
Limei HE
;
Shumei CAI
;
Tianhong ZHOU
Author Information
1. College of Life Science &Technology, Ji'an University, Guangzhou 510632, China. gyq69@163.com
- Publication Type:Journal Article
- MeSH:
Antineoplastic Agents;
chemistry;
immunology;
pharmacology;
Apoptosis;
drug effects;
Cell Proliferation;
drug effects;
Drug Synergism;
HeLa Cells;
Humans;
Interferon-gamma;
chemistry;
pharmacology;
Photochemistry;
Recombinant Proteins;
Tumor Necrosis Factor-alpha;
chemistry;
pharmacology
- From:
Journal of Biomedical Engineering
2006;23(6):1303-1307
- CountryChina
- Language:Chinese
-
Abstract:
To explore the effect of the co-photo-immobilization of Recombinant Human Interferon-r (IFN-gamma) and Recombinant Tumor Necrosis Factor (TNF-alpha) on the Hela cell cultivated in vitro. Co-immobilize the photoactive IFN-gamma and TNF-alpha on the polystyrene membrane; Cultivate the Hela cell on the cell culture polystyrene plate surface and set up the cultivation of the photo-immobilization IFN-gamma+TNF-alpha, Photo-immobilization IFN-gamma, nature (IFN-gamma, TNF-alpha, IFN-gamma+TNF-alpha). After 48 hours, the inhibition effect against Hela cells was studied in vitro by SEM and flow cytometry. It showed that the co-immobilization IFN-gamma+TNF-alpha has a more obvious inhibition effect on the Hela cell. And IFN-gamma can cooperate with TNF-alpha obviously.
