Study on allergenicity of fresh HAM for type I hypersensitivity.
- Author:
Min ZHAO
1
;
Qi ZHANG
;
Weixi CAO
;
Yongzhen TENG
;
Xiaoping ZHANG
;
Ke HU
;
Jing LU
;
Yingxiang QING
Author Information
1. Department of Ophthalmology, the First Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400016, China. minzhao2002@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Allergens;
immunology;
Amnion;
immunology;
Animals;
Guinea Pigs;
Histamine;
blood;
Humans;
Hypersensitivity, Immediate;
immunology;
Materials Testing
- From:
Journal of Biomedical Engineering
2006;23(6):1328-1331
- CountryChina
- Language:Chinese
-
Abstract:
To investigate whether human amniotic membrane (HAM) preparations have the possibility to type I hypersensitivity and its allergenicity. In systemic active allergic test model, 30 guinea pigs were equally divided into three groups. Each 10 guinea pigs were immunized with fresh HAM homogenate, albumen solution (positive control) and PBS (negative control). After the animals were stimulated with corresponding allergen, observe their reaction till dying or 3 h, then obtain blood samples, to determine blood histamine concentrations using chemical fluorometry and four hemorheologic markers by hemorheology analysis system. The guinea pigs responded to fresh HAM homogenate in almost the same manner as to PBS, and no obvious allergic reaction was observed in the animals except those in positive control group. The blood histamine concentration and four hemorheologic markers showed no significant differences between HAM and PBS (P > 0.05), both were much lower than positive control group (P < 0.01). Fresh HAM won't lead to type I hypersensitivity for lack of allergen performance.
