Characteristics of MSCs adhesion to polypeptides modified surface polymer PLGA-ASP-PEG.
- Author:
Dazhi YANG
1
;
Qixin ZHENG
;
Xiaodong GUO
;
Jie HAO
;
Yulin SONG
Author Information
1. Department of Orthopedics, Shenzhen Nanling Hospital, Shenzhen 518123, China. qpopop1977@163.com
- Publication Type:Journal Article
- MeSH:
Animals;
Animals, Newborn;
Aspartic Acid;
chemistry;
Biocompatible Materials;
chemistry;
Bone Marrow Cells;
cytology;
drug effects;
Cell Adhesion;
drug effects;
Cells, Cultured;
Female;
Lactic Acid;
chemistry;
Male;
Mesenchymal Stromal Cells;
cytology;
Peptides;
chemistry;
Polyglycolic Acid;
chemistry;
Rats;
Rats, Sprague-Dawley;
Tissue Engineering
- From:
Journal of Biomedical Engineering
2007;24(1):71-75
- CountryChina
- Language:Chinese
-
Abstract:
In this study we examined the in vitro characteristics of MSCs adhesion to polypeptides modified surface of polymer PLGA-[ASP-PEG]. We study the adhesion of marrow stromal cells in biomaterials at diferrent times using a micropipette aspiration technique. Comparison the adhesion of PLGA-[ASP-PEG] combinating GRGDSPC polypeptides versus PLGA-[ASP-PEG]. The adhesive conditions of MSCs on the materials were observed by scanning electron microscope. Four hours after MSCs inoculating in biomerials, the cell adhesion force of PLGA-[ASP-PEG] is 172.78 +/- 15. 23N and the force of PLGA-[ASP-PEG] combinating GRGDSPC polypeptides is 209.47 +/- 92.59N. There are no difference between two biomaterials. After 12 hours,the adhesion force of PLGA-[ASP-PEG] combinating GRGDSPC polypeptides is 576.23 +/- 165.74N, and the cell force of PLGA-[ASP-PEG] is 261.84 +/- 100.09 N. There are very significant difference between the two biomaterials. However, after 24 hours,the adhesion forces of the two biomaterials have no difference. The density of MSCs on PLGA-[ASP-PEG]-GRGDSPC surface was much higher than that of PLGA-[ASP-PEG]. Combination polypeptides in the surface of biomaterials can enhance the adhesion of MSCs.
