uPA expression of gastric cancer cell lines and its correlation with peritoneal seeding.
- Author:
You-Cheng DING
1
;
Zheng-Gang ZHU
;
Bing-Ya LIU
;
Yu-Bao JI
;
Xue-Hua CHEN
;
Yi ZHANG
;
Ying-Yan YU
;
Yan-Zhen LIN
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; enzymology; secondary; Animals; Cell Line, Tumor; Humans; Male; Mice; Mice, Inbred BALB C; Mice, Nude; Neoplasm Seeding; Peritoneal Neoplasms; secondary; RNA, Messenger; biosynthesis; genetics; Stomach Neoplasms; enzymology; pathology; Urokinase-Type Plasminogen Activator; biosynthesis; genetics
- From: Chinese Journal of Oncology 2005;27(1):13-15
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the correlation between expression of urokinase-type plasminogen activator (uPA) and capability of tumor cell seeding to the peritoneal membrane by different gastric cancer lines.
METHODSExpression of uPA in 4 human gastric cancer cell lines was examined by semi-quantitative RT-PCR, ELISA and Western blot. uPA activity was determined by an assay kit. After ip inoculation of cancer cells to nude mice, tumors on peritoneal membrane was grossly examined for tumor cell seedings.
RESULTSSGC7901 was the highest in uPA expression among human gastric cancer cell lines AGS, SGC7901, MKN45, and MKN28. MKN45 had the strongest uPA activity, while AGS was lowest in both uPA expression and activity. Peritoneal seeding tumors of various sizes were observed in mice inoculated with SGC7901 and MKN45 cells. In addition to peritoneal seedings, bloody ascites was present in mice inoculated with MKN28. The MKN45-inoculated mice took the least time to develop tumors and had the shortest surviving period. No peritoneal seeding was seen in mice inoculated with AGS cells.
CONCLUSIONThree of 4 human gastric cancer cell lines studied express uPA mRNA and activity, which correlate with their peritoneal seeding potentials.
