Expressions of VEGF/VEGFRs and activation of STATs in ovarian carcinoma.
- Author:
Bing-Ya CHEN
1
;
Da-Feng YE
;
Xing XIE
;
Huai-Zeng CHEN
;
Wei-Guo LÜ
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Cystadenocarcinoma, Mucinous; metabolism; pathology; Cystadenocarcinoma, Serous; metabolism; pathology; Cystadenoma, Mucinous; metabolism; pathology; Cystadenoma, Serous; metabolism; pathology; DNA-Binding Proteins; metabolism; Endothelial Cells; metabolism; Female; Humans; Middle Aged; Milk Proteins; metabolism; Ovarian Neoplasms; metabolism; pathology; Ovary; metabolism; STAT3 Transcription Factor; STAT5 Transcription Factor; Signal Transduction; Trans-Activators; metabolism; Vascular Endothelial Growth Factor A; metabolism; Vascular Endothelial Growth Factor Receptor-1; metabolism; Vascular Endothelial Growth Factor Receptor-2; metabolism
- From: Chinese Journal of Oncology 2005;27(1):33-37
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the expressions of VEGF/VEGFRs and activation of STATs in ovarian epithelial carcinoma, and to elucidate direct effect of VEGF on ovarian carcinoma cells.
METHODSTissue samples from 42 women with primary ovarian epithelial carcinoma (OVCA), 29 with begnin ovarian tumor (OVBT) and 11 with normal ovarian tissue (NOV) were collected. LSAB immunohistochemical staining was used to determine the expression of VEGF, VEGFR1, VEGFR2 and activated STATS (P-STAT1, P-STAT3, P-STAT5, P-STAT6) proteins.
RESULTS(1) Semi-quantitative scoring showed that VEGF expression in OVCA was significantly higher than that in OVBT and NOV (P < 0.01). Expressions of VEGFR1 and VEGFR2 were significantly elevated in OVCA, including tumor cells and stromal vascular endothelial cells (P < 0.01, compared with OVBT and NOV). There was no difference in VEGFRs expressions between OVBT and NOV. (2) In OVCA, tumor cells and endothelial cells expressed P-STAT3 and P-STAT5 at significantly higher levels than those in OVBT and NOV (P = 0.000). The staining of P-STAT1 and P-STAT6 was weak with no significant differences among OVCA, OVBT and NOV. (3) Expressions of VEGFR1 and VEGFR2 in endothelial cells were significantly correlated with P-STAT5 and P-STAT3, respectively (P = 0.006 and 0.001). In cancer cells, VEGF, VEGFR1 and VEGFR2 were all significantly correlated with P-STAT3 and P-STAT5 (P = 0.000), but not with P-STAT1 or P-STAT6.
CONCLUSIONVEGF affects ovarian carcinoma cells via VEGFRs, and STATs probably participate in intracellular signaling of VEGF.
