Analysis of BRCA1 mutations in Chinese patients with early onset breast cancer and affected relatives.
- Author:
Zhen HU
1
;
Jiong WU
;
Jin-song LU
;
Jian-min LUO
;
Qi-xia HAN
;
Zhen-zhou SHEN
;
Zhi-min SHAO
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Age of Onset; BRCA1 Protein; genetics; Breast Neoplasms; genetics; China; DNA Mutational Analysis; DNA, Neoplasm; blood; Exons; Female; Genes, BRCA1; Humans; Leukocytes, Mononuclear; chemistry; Mutation; Polymerase Chain Reaction; Polymorphism, Single-Stranded Conformational
- From: Chinese Journal of Oncology 2004;26(11):657-659
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the BRCA1 mutations in Chinese patients with early onset breast cancer and affected relatives.
METHODSGenomic DNA from 41 patients' peripheral blood mononuclear cells was studied by using polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) and DNA sequencing. BRCA1 mutations were detected in the whole gene sequence.
RESULTSDisease-related BRCA1 mutations were detected in 3 of the 41 patients studied. Two of the 3 patients were younger than 35 years old, and one patient with family history.
CONCLUSIONThe incidence of BRCA1 mutations in Chinese patients with early onset breast cancer is similar to that reported from western countries, but the incidence of mutations in Chinese breast cancer patients with affected relatives is comparatively low.
