Telomerase RNA antisense oligonucleotides inhibit growth of human choriocarcinoma xenograft in nude mice.
- Author:
Li-li CHEN
1
;
Wei ZHENG
;
Xue-jun CHEN
;
Liang WAN
;
Yi-fu SHI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibiotics, Antineoplastic; pharmacology; Cell Line, Tumor; Choriocarcinoma; enzymology; pathology; DNA-Binding Proteins; metabolism; Dactinomycin; pharmacology; Dose-Response Relationship, Drug; Female; Humans; Mice; Mice, Inbred BALB C; Mice, Nude; Neoplasm Transplantation; Oligonucleotides, Antisense; administration & dosage; pharmacology; Pregnancy; Telomerase; genetics; metabolism; Uterine Neoplasms; enzymology; pathology
- From: Chinese Journal of Oncology 2005;27(2):90-92
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the inhibitory effect of antisense oligonucleotides against telomerase RNA on the growth of human choriocarcinoma transplant in nude mice.
METHODSChoriocarcinoma xenografts were established by transplanting JAR cells subcutaneously to female nude mice, and were treated with high and low doses of antisense oligonucleotides. Control groups were treated with NS, random sequence and actinomycin D (Act-D). Tumor growth was monitored once every other day. Telomerase relative activity was assayed by TRAP-ELISA. Western blotting was used to detect expression of hTERT.
RESULTSLow and high doses antisense oligonucleotides, and Act-D inhibited tumor growth by 76.6%, 93.8% and 85.4% respectively, which were significantly different when compared with random sequence and NS groups. Expression of telomerase relative activity and hTERT were decreased as well. But the differences among the first three groups had no significance.
CONCLUSIONTelomerase RNA antisense oligonucleotide inhibits growth of human choriocarcinoma xenografts in nude mice. It may be a novel approach to the treatment of choriocarcinoma.
