Screening of differentially expressed genes in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis.
- Author:
Xiao-nan CUI
1
;
Jian-wu TANG
;
Li HOU
;
Bo SONG
;
Ji-wei LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Carcinoma, Hepatocellular; genetics; pathology; Cell Line, Tumor; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Gene Library; Liver Neoplasms, Experimental; genetics; pathology; Lymphatic Metastasis; genetics; Mice; Mice, Inbred Strains; Nucleic Acid Hybridization; methods; RNA, Messenger; genetics
- From: Chinese Journal of Oncology 2005;27(3):138-140
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo screen genes differentially expressed in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis.
METHODSA subtracted cDNA library of mouse hepatocarcinoma cell line with high potential of lymphatic metastasis Hca-F and its synogenetic cell line Hca-P with low metastatic potential was constructed by suppression subtractive hybridization (SSH) method. The screened clones of the subtracted library were sequenced and GenBank homology search was performed.
RESULTSTen differentially expressed cDNA fragments of Hca-F with high potential of lymphatic spreading were obtained, two of which were newly identified ones.
CONCLUSIONSSH is a useful technique to detect genes of differential expression and an effective method to clone novel genes.
