Effect of IL-24 gene expression on the growth of glioma cells in vitro and in vivo.
- Author:
Wen-ling LI
1
;
Bao-en SHAN
;
Yun-li YAN
;
Li-fen ZHENG
;
Qiao-xia LI
;
Na-jing ZHOU
;
Wen-qing ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Brain Neoplasms; metabolism; pathology; Cell Line, Tumor; Cell Proliferation; Genetic Vectors; Glioma; metabolism; pathology; Interleukins; biosynthesis; genetics; Male; Neoplasm Transplantation; Rats; Rats, Sprague-Dawley; Recombinant Proteins; biosynthesis; genetics; Retroviridae; genetics
- From: Chinese Journal of Oncology 2005;27(3):141-144
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of IL-24 expression on the growth of glioma cells.
METHODSThe IL-24 gene was transfected into rat glioma C6 cells with a retroviral vector. The expression of IL-24 in C6/IL-24 glioma cells was confirmed by RT-PCR. MTT assay and flow cytometry were used to study tumor cell proliferation in vitro. Tumorigenicity in vivo was studied in inbred SD male rats by the growth of intracerebrally inoculated tumor.
RESULTSIt was confirmed by RT-PCR that the exogenous IL-24 gene expressed in C6/IL-24 cell. The C6/IL-24 cell proliferation in vitro and tumorigenicity in vivo were both inhibited compared with its parental C6 cell.
CONCLUSIONIL-24 expression in glioma cells somehow inhibits their growth in vitro and in vivo.
