Spectrum of gene expression of a multi-drug resistant leukemia cell line with high tumorigenicity in nude mice.
- Author:
Li CHEN
1
;
Xiao-ping XU
;
Jian-min WANG
;
Yao LI
;
Hong XU
;
Shu-qing LÜ
;
Xin-hong FEI
;
Zheng-xia HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Drug Resistance, Multiple; genetics; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; genetics; Humans; K562 Cells; Mice; Mice, Nude; Oligonucleotide Array Sequence Analysis; Vincristine; pharmacology
- From: Chinese Journal of Oncology 2005;27(4):196-200
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the mechanism of multi-drug resistance of K562-n/VCR cell line with both bcr-abl and mdr-1 expressions by clustering analysis of differential gene expression profiles.
METHODSBy DNA microarray technique, genes differentially expressed by K562-n/VCR and K562-n cell lines were identified and analyzed.
RESULTSDNA microarray analysis of K562-n/VCR and K562-n cells was repeated three times and revealed 58 genes significantly differentially expressed among 12,800 genes arrayed. All but one was up-regulated in K562-n/VCR cells. The only gene down-regulated was a-myb. The up-regulated genes were MDR-associated genes, oncogenes, cytoskeleton, protein kinases and phosphatases, apoptotic and antiapoptotic factors, metabolism, transcriptional regulators associated with stress response, cell cycle checkpoint control, and genes for signal transduction proteins.
CONCLUSIONThese results indicate that, besides MDR-associated genes, other known and unknown genes may also be involved in the mechanism of multi-drug resistance.
