The function of ERK on Fas-mediated apoptotic signaling pathway of human gastric carcinoma cells.
- Author:
Ning LIU
1
;
Li-guang SUN
;
Hui-ying YU
;
Yi ZHANG
;
Qing YANG
;
Xiao-peng WANG
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; physiology; Cell Line, Tumor; Extracellular Signal-Regulated MAP Kinases; antagonists & inhibitors; metabolism; Fas Ligand Protein; metabolism; Humans; Signal Transduction; Stomach Neoplasms; pathology
- From: Chinese Journal of Oncology 2005;27(4):201-203
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the expression and functions of ERK (extracellular regulated kinase) in Fas-mediated apoptosis in gastric carcinoma cell line SGC-7901 and to elucidate the potential significance of this signaling pathway in tumor progression.
METHODSRadioisotope labeling and Western blotting with special anti-ERK antibody were used to check ERK activity in SGC-7901 cell line after anti-Fas antibody treatment. Apoptosis induced by several treatment factors was evaluated by FACS can flow cytometer.
RESULTSERK activity increased and reached the peak at 30 min after treatment with anti-Fas antibody and decreased in PD98059 pretreated group. The number of sub-G(1) cell was 30.5% +/- 2.6% in PD98059 pretreated group, which was higher than anti-Fas treatment group and control group, respectively.
CONCLUSIONIn gastric cancer cell line SGC-7901, Fas-induced ERK activation may suppress Fas-mediated apoptosis. Inhibition of ERK may enhance the sensitivity of SGC-7901 cells to Fas-mediated apoptosis. Fas-induced ERK activation may confer gastric cancer cells ability to escape the immune surveillance.
