The association of HeLa cell apoptosis induced by arsenic trioxide with the down-regulation of HPV18 E6 oncogene and inhibition of telomerase activity.
- Author:
Xiu-ning LU
1
;
Jie ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; pharmacology; Apoptosis; drug effects; Arsenicals; pharmacology; DNA-Binding Proteins; biosynthesis; genetics; Down-Regulation; HeLa Cells; Humans; Oncogene Proteins, Viral; biosynthesis; genetics; Oxides; pharmacology; Telomerase; metabolism
- From: Chinese Journal of Oncology 2005;27(5):265-268
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of As2O3 induction of HeLa cell apoptosis on HPV18 E6 expression and telomerase activity.
METHODSHeLa cells were treated with As2O3 in various concentrations. The effect of As2O3 on HeLa cell survival and apoptosis was determined by MTT assay, light and electron microscopy, and flow cytometry. Telomerase activity in HeLa cells was determined by TRAP-ELISA and the expression of HPV18 E6 mRNA was assayed by RT-PCR.
RESULTSAfter being treated with 2 micromol/L As2O3 for 48 h, the survival of HeLa cells decreased, and marked apoptosis was observed in a time- and dose-dependent manner. There was a good correlation between cell apoptosis and viral E6 gene expression and inhibition of telomerase activity following As2O3 treatment.
CONCLUSIONThe molecular mechanisms of As2O3 effect on HeLa cells may be related to down-regulation of HPV18 E6 oncogene expression and inhibition of telomerase activity.
