Induction of apoptosis in human hepatoma cell line SMMC7721 by Newcastle disease virus HN gene.
- Author:
Ying-chun SUN
1
;
Ning-yi JIN
;
Zhi-qiang MI
;
Xiao LI
;
Hai LIAN
;
Ping LI
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; physiology; Cancer Vaccines; immunology; Carcinoma, Hepatocellular; pathology; Caspase 3; metabolism; Cell Line, Tumor; HN Protein; genetics; Humans; Liver Neoplasms; pathology; Newcastle disease virus; genetics; Transfection; Vaccines, DNA; immunology
- From: Chinese Journal of Oncology 2005;27(5):279-282
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the mechanisms of apoptosis induced in human hepatoma cell line SMMC7721 by plasmid pVHN constructed with Newcastle disease virus (NDV) HN gene.
METHODSTwenty-four h after transfection with liposome-plasmid pVHN complexes in vitro, the mortality rate of SMMC7721 cells was determined by MTT staining and flow cytometry (FCM) with PI staining. The alteration of mitochondrial trans-membrane potential of the cells was detected by FCM with rhodamine 123 staining. Cell genomic DNA was detected by agarose electrophoresis. The activation of caspase-3 was assayed by its substrate color reaction.
RESULTSSignificant apoptosis was induced by transfection with plasmid pVHN into the cells for 24 h and the mortality rate was 50.0% (the mortality rate of control group was 5.2%). Genomic DNA was fragmented and mitochondrial trans-membrane potential was decreased, but caspase-3 activity increased.
CONCLUSIONSignificant apoptosis in SMMC7721 cells can be induced by NDV HN gene. Apoptosis may be resulted from the decrease of mitochondrial trans-membrane potential and activation of Caspase-3.