Inhibitory effects of roscovitine on proliferation and migration of vascular smooth muscle cells in vitro.
10.1007/s11596-014-1354-5
- Author:
Shuang-shuang ZHANG
1
;
Wei WANG
;
Chong-qiang ZHAO
;
Min-jie XIE
;
Wen-yu LI
;
Xiang-li YANG
;
Jia-gao LV
Author Information
1. Department of Cardiovascular Medicine, Huazhong University of Science and Technology, Wuhan, 430030, China, shuang5395@126.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Cycle;
drug effects;
Cell Line;
Cell Movement;
drug effects;
Graft Occlusion, Vascular;
drug therapy;
metabolism;
pathology;
Muscle, Smooth, Vascular;
metabolism;
pathology;
Myocytes, Smooth Muscle;
metabolism;
pathology;
Protein Kinase Inhibitors;
pharmacology;
Purines;
pharmacology;
Rats
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2014;34(6):791-795
- CountryChina
- Language:English
-
Abstract:
Abnormal proliferation and migration of vascular smooth muscle cells (VSMCs) are the major cause of in-stent restenosis (ISR). Intervention proliferation and migration of VSMCs is an important strategy for antirestenotic therapy. Roscovitine, a second-generation cyclin-dependent kinase inhibitor, can inhibit cell cycle of multiple cell types. We studied the effects of roscovitine on cell cycle distribution, proliferation and migration of VSMCs in vitro by flow cytometry, BrdU incorporation and wound healing assay, respectively. Our results showed that roscovitine increased the proportion of G0/G1 phase cells after 12 h (69.57±3.65 vs. 92.50±1.68, P=0.000), 24 h (80.87±2.24 vs. 90.25±0.79, P=0.000) and 48 h (88.08±3.86 vs. 88.87±2.43, P=0.427) as compared with control group. Roscovitine inhibited proliferation and migration of VSMCs in a concentration-dependent way. With the increase of concentration, roscovitine showed increased capacity for growth and migration inhibition. Roscovitine (30 μmol/L) led to an almost complete VSMCs growth and migration arrest. Combined with its low toxicity and selective inhibition to ISR-VSMCs, roscovitine may be a potential drug in the treatment of vascular stenosis diseases and particularly useful in the prevention and treatment of ISR.
