An LC-MS/MS method for the simultaneous determination of lycorine and galanthamine in rat plasma and its application to pharmacokinetic study of Lycoris radiata extract in rats.
10.1007/s11596-014-1365-2
- Author:
Xin ZHOU
1
;
Yue-bin LIU
;
Shan HUANG
;
Ying LIU
Author Information
1. Department of Pharmacy, Fuzhou General Hospital of Nanjing Military Command, Fuzhou, 350025, China, 13959188386@163.com.
- Publication Type:Journal Article
- MeSH:
Amaryllidaceae Alkaloids;
pharmacokinetics;
Animals;
Chromatography, Liquid;
Galantamine;
pharmacokinetics;
Lycoris;
chemistry;
Male;
Parasympathomimetics;
pharmacokinetics;
Phenanthridines;
pharmacokinetics;
Plant Extracts;
chemistry;
pharmacokinetics;
pharmacology;
Rats;
Rats, Wistar;
Tandem Mass Spectrometry;
methods
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2014;34(6):861-868
- CountryChina
- Language:English
-
Abstract:
A rapid, sensitive, and selective liquid chromatography-tandem mass spectrometry was developed for the simultaneous determination of lycorine and galanthamine, two major constituents in Lycoris radiata extract, in rat plasma. Liquid-liquid extraction with ethyl ether was carried out using diphenhydramine as the internal standard. The two bioactive alkaloids were separated on a Zorbax SB-C18 reserved-phase column (150 mm × 4.6 mm, i.d., 5 μm) by gradient elution using a mobile phase consisting of methanol with 0.1% formic acid (A) and water with 0.1% formic acid (B) at a flow rate of 0.6 mL/min. All analytes showed good linearity over a wide concentration range (r (2)>0.99) and the lower limit of quantification was 3.00 ng/mL for each analyte. The average extraction recovery of the analytes from rat plasma was more than 82.15%, and the intra-day and inter-day accuracy and precision of the assay were less than 12.6%. The validated method was successfully applied to monitoring the concentrations and pharmacokinetic studies of two Amaryllidaceous alkaloids in rat plasma after an oral administration of Lycoris radiata extract.
