miRNA-155 modulates the malignant biological characteristics of NK/T-cell lymphoma cells by targeting FOXO3a gene.
10.1007/s11596-014-1368-z
- Author:
Wei-guo JI
1
;
Xu-dong ZHANG
;
Xiang-dong SUN
;
Xiang-qi WANG
;
Bao-ping CHANG
;
Ming-zhi ZHANG
Author Information
1. Department of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China, demoben@126.com.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
genetics;
Forkhead Box Protein O3;
Forkhead Transcription Factors;
genetics;
metabolism;
Gene Expression Regulation, Neoplastic;
Humans;
Jurkat Cells;
Lymphoma, T-Cell;
genetics;
metabolism;
pathology;
MicroRNAs;
biosynthesis;
genetics;
Natural Killer T-Cells;
metabolism;
pathology;
Neoplasm Proteins;
genetics;
metabolism;
RNA, Neoplasm;
biosynthesis;
genetics;
Transduction, Genetic
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2014;34(6):882-888
- CountryChina
- Language:English
-
Abstract:
This study investigated the effects of miRNA-155 on malignant biological characteristics of NK/T-cell lymphoma cell lines and the possible mechanism. The expression of miRNA-155 was detected in lymphoma cell lines from different sources (SNK-6, YTS, Jurkat and DOHH2) by real-time PCR. Lentiviral vectors (pLL3.7) that could overexpress or downexpress miRNA-155 were constructed. Recombinant lentiviral particles were prepared and purified, and their titers determined. The expression of miRNA-155 in the infected SNK-6 cells and the cell proliferation were detected by PCR and CCK-8, respectively. Flow cytometry was used to determine the apoptosis of infected SNK-6 cells. The target of miRNA155 was predicted from Targetscan website. The effect of miRNA155 on FOXO3a expression was examined by Western blotting. The results showed that among the human NK/T-cell lymphoma cell lines SNK-6, YTS, Jurkat and DOHH2, the expression of miRNA-155 was highest in SNK-6. The infection efficiency of the recombinant lentivirus in SNK-6 was more than 70% at multiplicity of infection (MOI) of 100. The expression of miRNA-155 was significantly increased in SNK-6 cells infected by lentivirus vectors with high expression of miRNA-155 (4 times higher than the control group), and profoundly decreased in those infected with lentiviruses with low expression of miRNA-155. The proliferation of letivirus-infected SNK-6 cells was decreased as the expression of miRNA-155 reduced. The apoptosis rate was increased with the reduction in the expression of miRNA-155. FOXO3a was found to be a possible target of miRNA155, as suggested by Targetscan website. Western blotting showed that the expression of FOXO3a was significantly elevated in SNK-6 cells with miRNA-155 inhibition. It was concluded that reduction in miRNA-155 expression can inhibit the proliferation of SNK-6 lymphoma cells and promote their apoptosis, which may be associated with regulation of FOXO3a gene.
