Effect of microRNA-101 on proliferation and apoptosis of human osteosarcoma cells by targeting mTOR.
10.1007/s11596-014-1369-y
- Author:
Song LIN
1
;
Nan-nan SHAO
;
Lei FAN
;
Xiu-cai MA
;
Fei-fei PU
;
Zeng-wu SHAO
Author Information
1. Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China, prolins126@126.com.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Bone Neoplasms;
genetics;
metabolism;
pathology;
Cell Line, Tumor;
Cell Proliferation;
Humans;
MicroRNAs;
genetics;
metabolism;
Neoplasm Proteins;
genetics;
metabolism;
Osteosarcoma;
genetics;
metabolism;
pathology;
RNA, Neoplasm;
genetics;
metabolism;
TOR Serine-Threonine Kinases;
genetics;
metabolism
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2014;34(6):889-895
- CountryChina
- Language:English
-
Abstract:
Studies have proved that microRNA-101 (miR-101) functions as a tumor suppressor and is associated with growth and apoptosis of various human cancers. However, the role of miR-101 in osteosarcoma and the possible mechanism by which miR-101 affects the tumor growth and apoptosis have not been fully elucidated. In this study, we found that the expression of miR-101 was down-regulated in osteosarcoma tissues and Saos-2 cell line as compared with that in adjacent non-neoplastic bone tissues and the osteoblastic cell line. To better characterize the role of miR-101 in osteosarcoma, we used a gain-of-function analysis by transfecting human osteosarcoma cell line Saos-2 with chemically synthesized miR-101 mimics. The results showed that overexpression of miR-101 inhibited the proliferation and promoted the apoptosis of Saos-2 cells. Meanwhile, bioinformatic analysis demonstrated that mTOR gene was a direct target of miR-101. Overexpression of miR-101 significantly decreased the expression of mTOR at both mRNA and protein levels in Saos-2 cells, consequently inhibiting Saos-2 cells proliferation and promoting cells apoptosis in an mTOR-dependent manner. Taken together, these data suggest that miR-101 may act as a tumor suppressor, which is commonly downregulated in both osteosarcoma tissues and cells. mTOR plays an important role in mediating miR-101 dependent biological functions in osteosarcoma. Reintroduction of miR-101 may be a novel therapeutic strategy by down-regulating mTOR expression.
