Effect of retinoic acid on expression of LINGO-1 and neural regeneration after cerebral ischemia.
10.1007/s11596-015-1388-3
- Author:
Hong-yi XING
1
;
Er-yan MENG
;
Yuan-peng XIA
;
Hai PENG
Author Information
1. Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China, xinghongyi@sina.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Blotting, Western;
Brain Ischemia;
metabolism;
GAP-43 Protein;
genetics;
metabolism;
Gene Expression;
drug effects;
Male;
Membrane Proteins;
genetics;
Nerve Tissue Proteins;
genetics;
Neurons;
drug effects;
metabolism;
Rats;
Rats, Sprague-Dawley;
Tretinoin;
pharmacology
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2015;35(1):54-57
- CountryChina
- Language:English
-
Abstract:
The purpose of this study was to observe the expression of LINGO-1 after cerebral ischemia, investigate the effects of retinoic acid (RA) on the expression of LINGO-1 and GAP-43, and the number of synapses, and to emplore the repressive effect of LINGO-1 on neural regeneration after cerebral ischemia. The model of permanent focal cerebral ischemia was established by the modified suture method of middle cerebral artery occlusion (MCAO) in Sprague-Dawley (SD) rats. The expression of LINGO-1 was detected by Western blotting and that of GAP-43 by immunohistochemistry. The number of synapses was observed by transmission electron microscopy. The SD rats were divided into three groups: sham operation (sham) group, cerebral ischemia (CI) group and RA treatment (RA) group. The results showed that the expression level of LINGO-1 at 7th day after MCAO in sham, CI and RA groups was 0.266 ± 0.019, 1.215 ± 0.063 and 0.702 ± 0.081, respectively (P<0.01). The number of Gap-43-positive nerve cells at 7th day after MCAO in sham, CI and RA group was 0, 59.08 ± 1.76 and 76.20 ± 3.12 per high power field, respectively (P<0.05). The number of synapses at 7th day after MCAO was 8.42 ± 0.13, 1.74 ± 0.37 and 5.39 ± 0.26 per μm², respectively (P<0.05). It is concluded that LINGO-1 expression is up-regulated after cerebral ischemia, and RA inhibits the expression of LINGO-1, promotes the expression of GAP-43 and increases the number of synapses. It suggests that LINGO-1 may be involved in the pathogenesis of cerebral ischemia, which may provide an experimenal basis for LINGO-1 antogonist, RA, for the treatment of cerebral ischemia.
