Decreased number of CD14+TLR4+ monocytes and their impaired cytokine responses to lipopolysaccharide in patients with chronic kidney disease.
10.1007/s11596-015-1412-7
- Author:
Zhi LIU
1
;
Yan-hong KAN
;
Yu-dan WEI
;
Xiu-jiang LI
;
Fan YANG
;
Yue HOU
;
Yu-jun DU
Author Information
1. Department of Nephrology, the First Hospital of Jilin University, Changchun, 130021, China, bojidb@163.com.
- Publication Type:Journal Article
- MeSH:
Cytokines;
metabolism;
Humans;
Kidney Failure, Chronic;
metabolism;
Lipopolysaccharide Receptors;
metabolism;
Lipopolysaccharides;
pharmacology;
Monocytes;
metabolism;
Toll-Like Receptor 4;
metabolism
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2015;35(2):206-211
- CountryChina
- Language:English
-
Abstract:
This study aimed to examine the number of circulating Toll-like receptor 4 (TLR4) + CD14+ monocytes in patients with different stages of chronic kidney disease (CKD), their responses to lipopolysaccharide (LPS), and to explore the potential association of the number of TLR4+CD14+ monocytes with clinical laboratory measures. The numbers of TLR4+CD14+, LPS-stimulated TNF-α+CD14+ and interleukin (IL)-6+CD14+ monocytes were determined by flow cytometry in 9 patients with stage 3 CKD, 11 with stage 4 CKD, 16 with stage 5 CKD, and 19 healthy controls (HCs). Their laboratory tests were performed by routine methods and the potential association among these measures was analyzed by Pearson's correlation analysis. The numbers of CD14+, CD14+TLR4+, LPSstimulated TNF-α+CD14+ and IL-6+CD14+ monocytes in patients with CKD were significantly less than those of HCs (all P<0.05), and were negatively associated with patient disease severity. The number of CD14+TLR4+ monocytes was positively correlated with estimated glomerular filtration rate (eGFR, P<0.001) and the levels of hematocrit (P<0.01), but negatively correlated with the levels of blood urine nitrogen, serum creatinine, and C-reactive protein (P<0.001 for all), in the CKD patients. Our data indicate that significant reduction in the number of TLR4+ monocytes and their impaired responses to LPS may be associated with the progression of CKD in Chinese patients.
