Effects of oxidized low density lipoprotein on transformation of valvular myofibroblasts to osteoblast-like phenotype.
10.1007/s11596-015-1438-x
- Author:
Di CHEN
1
;
Ying-Lian SHEN
;
Wei-Lin HU
;
Zheng-Ping CHEN
;
Yong-Sheng LI
Author Information
1. Department of Emergency Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China, chendi8073@163.com.
- Publication Type:Journal Article
- MeSH:
Actins;
metabolism;
Animals;
Aortic Valve;
cytology;
Cell Differentiation;
drug effects;
Cells, Cultured;
Gene Expression Regulation;
drug effects;
Intercellular Signaling Peptides and Proteins;
pharmacology;
Lipoproteins, LDL;
pharmacology;
Myofibroblasts;
drug effects;
Osteoblasts;
physiology;
Phenotype;
Swine;
Wnt Signaling Pathway;
drug effects;
beta Catenin;
metabolism
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2015;35(3):362-367
- CountryChina
- Language:English
-
Abstract:
In order to investigate the roles of Wnt signal pathway in transformation of cardiac valvular myofibroblasts to the osteoblast-like phenotype, the primary cultured porcine aortic valve myofibroblasts were incubated with oxidized low density lipoprotein (ox-LDL, 50 mg/L), and divided into four groups according to the ox-LDL treatment time: control group, ox-LDL 24-h group, ox-LDL 48-h group, and ox-LDL 72-h group. Wnt signal pathway blocker Dickkopf-1 (DDK-1, 100 μg/L) was added in ox-LDL 72-h group. The expression of a-smooth muscle actin (α-SMA), bone morphogenetic protein 2 (BMP2), alkaline phosphatase (ALP), and osteogenic transcription factor Cbfa-1 was detected by Western blotting, and that of β-catenin, a key mediator of Wnt signal pathway by immunocytochemical staining method. The Wnt/β-catenin was observed and the transformation of myofibroblasts to the osteoblast-like phenotype was examined. The expression of α-SMA, BMP2, ALP and Cbfa-1 proteins in the control group was weaker than in the ox-LDL-treated groups. In ox-LDL-treated groups, the protein expression of a-SMA, BMP2, ALP, and Cbfa-1 was significantly increased in a time-dependent manner as compared with the control group, and there was significant difference among the three ox-LDL-treated groups (P<0.05 for all); β-catenin protein was also up-regulated in the ox-LDL-treated groups in a time-dependent manner as compared with the control group (P<0.05), and its transfer from cytoplasm to nucleus and accumulation in the nucleus were increased in the same fashion (P<0.05). After addition of DKK-1, the expression of α-SMA, bone-related proteins and β-catenin protein was significantly reduced as compared with ox-LDL 72-h group (P<0.05). The Wnt/ β-catenin signaling pathway may play an important role in transformation of valvular myofibroblasts to the osteoblast-like phenotype.
