Application of multiplex nested RT-PCR for fast detection of PDGFRα fusion gene in myeloproliferative neoplasms.
- Author:
Meng-Meng JIANG
1
;
Min-Hang ZHOU
;
Li GAO
;
Yi DING
;
Yuan-Yuan XU
;
Li-Li WANG
;
Yu JING
;
Quan-Shun WANG
;
Li YU
Author Information
1. Department of Hematology, Chinese PLA General Hospital, Beijing, China.
- Publication Type:Journal Article
- MeSH:
Bone Marrow Neoplasms;
diagnosis;
genetics;
Gene Fusion;
Humans;
Multiplex Polymerase Chain Reaction;
Myeloproliferative Disorders;
diagnosis;
genetics;
Receptor, Platelet-Derived Growth Factor alpha;
genetics;
Reverse Transcriptase Polymerase Chain Reaction;
methods
- From:
Journal of Experimental Hematology
2011;19(6):1447-1449
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to explore the applicable value of multiplex nested reverse transcription-polymerase chain reaction (multiplex nested RT-PCR)for the detection of platelet-derived growth factor receptor alpha (PDGFRα) fusion gene in myeloproliferative neoplasms (MPN). Bone marrow or peripheral blood samples from 146 patients with MPN were analyzed by using a novel multiplex nested RT-PCR. The result showed that PDGFRα fusion gene was found in 6 out of the 146 bone marrow or peripheral blood samples, the positive rate was 4.11%, 4 from the 6 patients received treatment with imatinib and showed therapeutic effect. It is concluded that the multiplex nested RT-PCR has a series of advantages such as high sensitivity, specificity, and time-saving, and can be applied for determination of the molecular type of MPN, and also for the diagnosis and therapy of MPN.
