Analysis of gene polymorphism of HPA and HLA-I in Chinese Xi'an voluntary platelet donors.
- Author:
Xiao-Fang WANG
1
;
Meng-Li LIU
;
Dang-Xia ZHOU
;
Jun QI
;
Sheng LIU
;
Tian-Ju WANG
Author Information
1. Blood Center of Shaanxi, Xi'an, Shaanxi Province, China. xinlvcheng2006@163.com
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Alleles;
Antigens, Human Platelet;
genetics;
Asian Continental Ancestry Group;
genetics;
Blood Donors;
China;
Female;
Genes, MHC Class I;
Humans;
Male;
Middle Aged;
Phenotype;
Young Adult
- From:
Journal of Experimental Hematology
2011;19(6):1462-1465
- CountryChina
- Language:Chinese
-
Abstract:
To study the allele frequencies and their polymorphism characteristics of human platelet antigen (HPA) and human leucocyte antigen-I (HLA-I) in Chinese xi'an population, the types of HPA and HLA-I in 375 Chinese xi'an voluntary platelet donors were detected by PCR-SSP and PCR-SSO as well as flow cytometry with magnetic beads, and were analyzed. The results showed that there was no polymorphism in HPA-7-HPA-14, HPA-16 and HPA-17 which only expressed-aa type, the -bb type was only detected in HPA-3 and HPA-15, 9 out of 16 samples for the HPA-5ab phenotype simultaneously expressed HPA-15ab, the other 7 samples expressed HPA-15bb, no HPA-15aa phenotype was observed. Phenotypes detected in this study were HPA-1aa-17aa, HPA-1ab, -2ab, -3ab, -3bb, -4ab, -5ab, -6ab, -15ab and -15bb. Among 375 cases, HLA-A specificity of 16 species was observed, which accounted for 76% (16/21) of detectable phenotype specificity in this locus, moreover, 11 species showed frequency > 1%; HLA-B specificity of 36 species was observed which accounted for 84% (36/43) of detectable phenotype specificity in this locus, moreover 23 species showed frequency > 1%, these species were covered by common specific HLA-I in northern China, 264 species haplotype HLA-A-B were found in 375 cases, the frequency of 30 species was > 1%. It is concluded that the gene frequency distribution of HPA and HLA-I in Chinese Xi'an population is in accordance with population of northern China on the whole, but it has its own characteristics.
