HLA-A, B and DRB1 polymorphism at high-resolution in Han population from southern area of Shandong province in China.
- Author:
Yi ZHANG
1
;
Xiang-Min NIE
;
Yun-Long ZHUANG
;
Yong-Hong SONG
;
Yan LIU
;
Chuan-Fu ZHU
Author Information
1. Blood Center of Shandong Province, Jinan, Shandong Province, China.
- Publication Type:Journal Article
- MeSH:
Alleles;
Asian Continental Ancestry Group;
genetics;
China;
Female;
Gene Frequency;
Genetics, Population;
HLA-A Antigens;
genetics;
HLA-B Antigens;
genetics;
HLA-DRB1 Chains;
genetics;
Haplotypes;
Humans;
Male;
Polymorphism, Genetic
- From:
Journal of Experimental Hematology
2011;19(6):1482-1488
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to analyze the polymorphism of HLA-A, B, DRB1 alleles at high-resolution level in Han population from southern area of Shandong province in China. 688 randomly selected, unrelated and healthy individual from southern area of Shandong province were genotyped for HLA-A, -B and HLA-DRB1 loci by PCR-SBT. Then, allelic and haplotypic distributions of HLA-A, B and DRB1 were estimated by maximum likelihood estimation method using Arlequin 3.0. The results indicated that a total of 31 HLA-A, 63 HLA-B and 39 HLA-DRB1 alleles were identified in Han Population from southern area of Shandong province. Six HLA-A alleles were found with a frequency greater than 0.05 (A*24:02, *30:01, *11:01, *02:01, *33:03 and *02:06), with a cumulative frequency of 0.7223. For HLA-B locus, there were also six alleles which had a frequency higher than 5% (B*1302, *4403, *5101, B*4601, *1501 and *5801), representing 0.4432 of the all alleles in the population. And four HLA-DRB1 alleles were defined as predominant (DRB1*0701, *1501, *0901and *0803), accounting for 0.5453 of the defined alleles. The most common three-loci haplotype was A*30:01-B*13:02-DRB1*07:01 (0.1151) and the most frequent two-loci haplotype were A*30:01-B*13:02 (0.1303), A*30:01-DRB1*07:01 (0.1157) and B*13:02-DRB1*07:01 (0.1307). It is concluded that the allelic and haplotypic diversities of HLA-A, -B and HLA-DRB1 at high-resolution in Han population from southern area of Shandong province in China provide useful information for HLA matching in transplantation and diseases-associated study in this population.
