Effects of interferon-α combined with homoharringtonine on K562 cell proliferation and β-catenin expression.
- Author:
Yu-Ye SHI
1
;
Wei-Ke CAO
;
Xiao-Ning LIU
;
Zhi-Kui DENG
;
Hua GUO
;
Wan-Ting FENG
;
Li-Lin YE
;
Jia-Bing ZHU
;
Yu-Feng LI
Author Information
1. Department of Hematology, Nanjing Medical University, Huaian, Jiangsu Province, China.
- Publication Type:Journal Article
- MeSH:
Cell Proliferation;
drug effects;
Harringtonines;
pharmacology;
Humans;
Interferon-alpha;
pharmacology;
K562 Cells;
beta Catenin;
genetics;
metabolism
- From:
Journal of Experimental Hematology
2012;20(1):43-47
- CountryChina
- Language:Chinese
-
Abstract:
The study was aimed to investigate the synergistically effect of interferon-α (IFN-α) and homoharringtonine (HHT) on the proliferation, apoptosis, cell cycle of K562 cells and the expression of β-catenin. The proliferation, apoptosis, cell cycle and β-catenin mRNA expression of K562 cells treated with IFN-α and/or HHT were assayed with MTT, flow cytometry or RT-PCR respectively. The results showed that HHT alone, but not IFN-α alone, displayed a proliferation inhibition, apoptosis induction, G(0)/G(1) phase block and down-regulation of β-catenin expression in K562 cells with concentration- and time-dependent manners. The expression level of β-catenin mRNA after being treated with HHT was 0.5576 ± 0.0373, which were lower than that in control group (0.9369 ± 0.0142). The down-regulation of β-catenin expression in group of IFN-α combined with HHT was higher significantly than that in HHT group (0.3737 ± 0.0529 vs 0.5576 ± 0.0373, P < 0.05). Otherwise, HHT combined with IFN-α did not demonstrate obvious toxicologic effect on bone marrow mononuclear cells. It is concluded that IFN-α combined with HHT can enhance the cytotoxic effect of HHT on K562 cells, which may be associated with down-regulation of β-catenin expression.
