Caspase-independent apoptosis induced by arsenic trioxide in human multiple myeloma cell RPMI8226.
- Author:
Jia XIE
1
;
Mei ZHANG
;
Yan-Ping SONG
;
Kai HU
;
Jing-Jing REN
;
Yun-Jie ZHANG
Author Information
1. Department of Hematology, Xi'an Jiaotong University Medical College, Shaanxi Province, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Arsenicals;
pharmacology;
Caspase 3;
metabolism;
Cell Line, Tumor;
Humans;
Multiple Myeloma;
metabolism;
pathology;
Oxides;
pharmacology;
Proto-Oncogene Proteins c-bcl-2;
metabolism
- From:
Journal of Experimental Hematology
2012;20(1):107-111
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to explore the caspase-independent apoptosis pathway in human multiple myeloma cell RPMI8226 induced by arsenic trioxide (As(2)O(3)). MTT method was used to analyze the proliferation inhibition rate; flow cytometry was used to detect the apoptosis rate; Western blot was used to determine the expressions of BCL-2 and Caspase-3 in RPMI8226 cells. The results showed that As(2)O(3) (0.1 - 20 µmol/L) significantly inhibited the proliferation of RPMI8226 (P < 0.05) in concentration- and time-dependent manner. Compared with the group treated with As(2)O(3) (10 µmol/L) alone, the apoptosis rate of zVAD-fmk (20 µmol/L) and As(2)O(3) combined treated group did not change. Compared with the group treated with As(2)O(3) (10 µmol/L) alone, zVAD-fmk (20 µmol/L) combined with As(2)O(3) (10 µmol/L) treatment group showed significant increase of expressions of Caspase-3 and BCL-2. It is concluded that As(2)O(3) can inhibit the proliferation of RPMI8226 cells. As(2)O(3) can induce apoptosis of RPMI8226 cells, and a caspase-independent process probably exist in As2O3-inducing RPMI8266 cells apoptosis.
