Discrimination of alleles in HLA-C*07:01:01G and HLA-C*07:02:01G groups through detection sequences in exons 1 to 7 of HLA-C locus by using polymerase chain reaction sequence-based typing.
- Author:
Hang-Jun LÜ
1
;
Wei ZHANG
;
Jun-Jun HE
;
Yan-Min HE
;
Wei WANG
;
Zhe-Dong HANG
;
Nan-Yin CHEN
;
Fa-Ming ZHU
;
Li-Xing YAN
Author Information
1. Institute of Transfusion Medicine, Blood Center of Zhejiang Province, Hangzhou, Zhejiang Province, China. lhj@zjb.org.cn
- Publication Type:Journal Article
- MeSH:
Alleles;
Base Sequence;
Exons;
HLA-B Antigens;
genetics;
HLA-C Antigens;
genetics;
Humans;
Molecular Sequence Data;
Polymerase Chain Reaction;
methods;
Sequence Analysis, DNA
- From:
Journal of Experimental Hematology
2012;20(1):178-181
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to discriminate the alleles in the HLA-C*07:01:01G and HLA-C*07:02:01G groups and analyze their associations with HLA-B locus. Samples previously typed as HLA-C*07:01:01G and HLA-C*07:02:01G were collected. The nucleotide sequences in exons 1 to 7 of the HLA-C locus were sequenced by polymerase chain reaction sequence-based typing (PCR-SBT) and HLA-B genotyping was also preformed by PCR-SBT in these samples. The results showed that 4 samples (30.8%) were confirmed as HLA-C*07:01:01 and 9 samples (69.2%) were HLA-C*07:06 among 13 samples previously typed as HLA-C*07:01:01G. Linkage disequilibrium (LD) analysis showed that HLA-C*07:06 allele was strongly related with HLA-B*44:03. All samples were typed as C*07:02:01 among 102 individuals previously typed as C*07:02:01G. LD analysis showed that C*07:02:01 was strongly related with HLA-B*51:01, B*46:01, B*39:01, B*40:01, B*38:02, B*15:02 alleles. It is concluded that HLA-C*07:01:01 and HLA-C*07:06 alleles are confirmed in the HLA-C*07:01:01G group and HLA-C*07:02:01 is a preferred allele in the HLA-C*07:02:01G.
