Effect of berberine on HL-60 cell proliferation, apoptosis and vascular endothelial growth factor receptor 2 expression.
- Author:
Shao-Zhu LIN
1
;
Ming-Zhen CHEN
;
Xiu-Lan HUANG
Author Information
1. Department of Pediatrics, Affliated Hospital of Guangdong Medical College, Zhanjiang, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Berberine;
pharmacology;
Cell Proliferation;
drug effects;
Flow Cytometry;
Gene Expression Regulation, Leukemic;
HL-60 Cells;
Humans;
Vascular Endothelial Growth Factor Receptor-2;
metabolism
- From:
Journal of Experimental Hematology
2012;20(2):262-267
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the effect of berberine on the proliferation and apoptosis of HL-60 cells, and the expression of vascular endothelial growth factor receptor 2 (VEGFR2) in HL-60 cells. Berberine (6 - 96 µg/ml) was added to the HL-60 cell line culture medium, the CCK-8 method was used to reveal the inhibitory effect on cell proliferation, the flow cytometry was used to determine the apoptosis rate and cell cycle in HL-60 cells treated with berberine. The expression of VEGFR2 mRNA and protein were examined by RT-PCR and Western blot respectively. The results showed that the berberine inhibited the proliferation of HL-60 cells and induced their apoptosis in dose- and time-dependent manners. With the increased concentration of berberine, the percentage of HL-60 cells in G(1) phase of cycle increased significantly, and the percentage of HL-60 cells in S phase decreased significantly. The expression of mRNA and protein of VEGFR2 decreased with the increased concentration of berberine. It is concluded that the berberine can inhibit HL-60 cell proliferation and induce HL-60 cell apoptosis. The expression of mRNA and proteins of VEGFR2 decreased after treatment with berberine.
