Component I from Agkistrodon acutus venom induces apoptosis of K562/A02 cells by promoting caspase 3 expression.
- Author:
Bing ZHOU
1
;
Gen-Bao ZHANG
;
Ting DUAN
;
Jue ZHOU
;
Juan WU
Author Information
1. Department of Pathophysioloy, Wuhu Medical College, Wuhu, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
drug effects;
Caspase 3;
metabolism;
Cell Proliferation;
drug effects;
Crotalid Venoms;
pharmacology;
Gene Expression Regulation, Leukemic;
Humans;
K562 Cells;
Leukemia;
metabolism;
pathology
- From:
Journal of Experimental Hematology
2012;20(2):273-276
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effects of component I from Agkistrodon acutus venom (AAVC-I) on the biological features of chronic myeloid leukemia cells, K562/A02 leukemia cells were cultured in the presence of AAVC-I (6.25 - 100 µg/ml) and the proliferation status was assayed by CCK-8 method. Morphological changes were observed by inversed microscope after Giemsa and Hochest 33258 staining, and cell apoptosis was detected by flow cytometry. Caspase 3 activity was tested by using Chromogenic Activity Assay Kit. The results showed that AAVC-I inhibited the growth of K562/A02 cells in time- and concentration-dependant manners, and the IC(50) at 48 h was 30.988 µg/ml. Giemsa and Hochest 33258 staining showed the typical apoptotic features in K562/A02 cells after induction with AAVC-I for 48 h. Flow cytometric analysis revealed that the percentage of the apoptotic cells reached from 0.88 up to 53.66 as the treated concentration was elevated from 0 to 50 µg/ml. Compared with the control group, the expression of caspase 3 in the tested group was enhanced in a dose-dependent manner (P < 0.05). It is concluded that AAVC-I can effectively inhibit the growth and promote apoptosis of K562/A02 cells. Elevated expression of caspase-3 may be attributed to the apoptosis of K562/A02 cells.
