Inhibitory effects of sporoderm-broken Ganoderma lucidum spores on growth of lymphoma implanted in nude mouse.
- Author:
Juan CHEN
1
;
Ying-Hao YU
Author Information
1. Department of Pathology, Fuzhou General Hospital, Fuzhou, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
Gene Expression Regulation, Neoplastic;
Lymphoma, T-Cell;
metabolism;
pathology;
Male;
Mice;
Mice, Inbred BALB C;
Mice, Inbred C57BL;
Mice, Nude;
Proto-Oncogene Proteins;
metabolism;
Proto-Oncogene Proteins c-bcl-2;
Reishi;
Spores, Fungal;
Thymus Neoplasms;
metabolism;
prevention & control;
Transplantation, Heterologous;
bcl-2-Associated X Protein;
metabolism;
bcl-X Protein;
metabolism
- From:
Journal of Experimental Hematology
2012;20(2):310-314
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the inhibitory effects of sporoderm-broken ganoderma lucidum spores (GLS) on transplanted lymphoma in nude mice and its mechanism. The models of the subcutaneously transplanting tumor were established by N-methylnitrosourea-induced thymus T-cell lymphoma. The cellular apoptosis of tumor tissues in nude mice were observed by transmission electron microscopy (TEM), the mRNA expression of Bax, Bcl-2, Bcl-xl was determined by RT-PCR. The results indicated that the GLS had a certain anti-tumor effect, and the inhibitory rate was 45.8 with the dose of 4 g/kg (ig, once daily for 21 d). Apoptotic cells in lymphoma cells treated with GLS were observed by TEM. RT-PCR analysis showed that the expression of Bax was up-regulated, Bcl-2 and Bcl-xl were down-regulated in T-cell lymphoma cells. It is concluded that GLS can inhibit proliferation of lymphoma cells and induce the lymphoma cell apoptosis, the mechanism of which may be related with up-regulating the expression of Bax and down-regulating the expression of Bcl-2 and Bcl-xl.
