Effects of triptolide on bortezomib-induced apoptosis in multiple myeloma cells.
- Author:
Yang YANG
1
;
Hong-Juan DONG
;
Guang-Xun GAO
;
Yi-Wei WANG
;
Hong-Tao GU
;
Xie-Qun CHEN
Author Information
1. Department of Geriatric Hematology, Chinese PLA General Hospital, Beijing, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Boronic Acids;
pharmacology;
Bortezomib;
Cell Line, Tumor;
Diterpenes;
pharmacology;
Epoxy Compounds;
pharmacology;
Humans;
Multiple Myeloma;
pathology;
Phenanthrenes;
pharmacology;
Pyrazines;
pharmacology
- From:
Journal of Experimental Hematology
2012;20(2):335-338
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to investigate the effect of triptolide on bortezomib-induced apoptosis in multiple myeloma cell line NCI-H929(H929). MTT assay was applied to detect the inhibitory effects of triptolide and bortezomib alone or combined at different concentrations on H929 cells, the cell apoptosis was assayed by flow cytometry with Annexin V-FITC/PI staining. The results showed that both triptolide (10 - 100 ng/ml) and bortezomib (10 - 100 nmol/L) alone or combination inhibited the proliferation of MM cell line H929 in a concentration-dependent manner. The apoptotic rate of H929 cells in group of triptolide combined with bortezomib was much higher than that in groups of single drug or control; moreover, the apoptotic rate of H929 cells treated by non-inhibitory concentration of triptolide (10 ng/ml) combined with bortezomib (40 nmol/L) for 24 h was significantly higher than that by bortezomib alone (P < 0.05). It is concluded that triptolide can significantly enhance the pro-apoptotic activity of bortezomib in MM cells.
