Correlation between cyclin A gene expression in adult patients with acute leukemia and drug resistance.
- Author:
Jie MA
1
;
Shirong XU
;
Yongrong LAI
;
Yuying LU
;
Ergu YAO
Author Information
1. Department of Hematology, Second Hospital, Hebei Medical University, Shijiazhuang 050000.
- Publication Type:Journal Article
- MeSH:
ATP-Binding Cassette, Sub-Family B, Member 1;
genetics;
Adolescent;
Adult;
Aged;
Cyclin A;
biosynthesis;
genetics;
Drug Resistance, Multiple;
genetics;
Drug Resistance, Neoplasm;
genetics;
Female;
Genes, MDR;
genetics;
Humans;
Leukemia, Myeloid, Acute;
genetics;
Leukemia, Promyelocytic, Acute;
genetics;
Male;
Middle Aged;
Precursor Cell Lymphoblastic Leukemia-Lymphoma;
genetics;
RNA, Messenger;
biosynthesis;
genetics
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2003;23(3):245-248
- CountryChina
- Language:English
-
Abstract:
In order to investigate the relationship between the expression of cyclin A and drug resistance in adult patients with acute leukemia (AL), the mRNA expression of cyclin A, mdr1, Top II alpha, bcl-2 was detected in 64 adult patients with AL and 20 normal controls by semi-reverse transcription polymerase chain reaction (semi-RT-PCR). It was found that the cyclin A and Top II alpha mRNA expression levels in drug resistant group were significantly lower than in sensitive group (P < 0.01). Under the same experimental condition no cyclin A mRNA expression was detectable in all normal controls. The mdr1 and bcl-2 mRNA expression levels in resistant group were significantly higher than in sensitive group (P < 0.01), cyclin A and Top II alpha gene expression levels were closely correlated (rs = +0.794, P = 0.000, n = 64) in all AL patients, but cyclin A was not correlated with mdr1 and bcl-2 gene expression levels. In drug resistant group there was a negative correlation between the gene expression levels of cyclin A and mdr1 (rs = -0.337, P = 0.029). The 10 AL patients with positive lower expression of both cyclin A and Top II alpha were all resistant to drugs. Logistic regression of Binary analysis showed the correlation between the lower expression of cyclin A and drug resistance. It was concluded that lower expression of cyclin A gene might be an unfavorable prognostic factor for patients with AL, and detection of both cyclin A and Top II alpha gene expression would predict drug resistance in AL patients.
