Observation on in situ hybridization and immunocytochemistry of matrix metalloproteinases in rat pancreas.
- Author:
Lihua TANG
1
;
Shenghong LIU
;
Fang WANG
;
Zilong LIU
;
Yun XU
;
Xiaoli WANG
;
Zhaochun LI
Author Information
1. Department of Histology and Embryology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Division;
Immunohistochemistry;
In Situ Hybridization;
Male;
Matrix Metalloproteinase 1;
analysis;
biosynthesis;
genetics;
Pancreas;
enzymology;
RNA, Messenger;
analysis;
biosynthesis;
genetics;
Rats;
Rats, Sprague-Dawley
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2003;23(4):332-334
- CountryChina
- Language:English
-
Abstract:
In situ hybridization and immunocytochemical techniques were employed to examine the expression of matrix metalloproteinases-1 (MMP-1) and to identify the pattern of its distribution in rat pancreas. The results indicated that the signal of MMP-1 mRNA and MMP-1 positive immunoreaction were detected in some fiberoblasts around interlobular ducts and exocrine cell in margin acinus of some lobules, but the signal of MMP-1 mRNA and MMP-1 positive immunoreaction could not be detected in most of other acinus and islets of pancreas. It is concluded that the expression of MMP-1 in above cells of rat might play an important role in acinar proliferation and differentiation of rat pancreatic tissues.
