A comparative study on the effect of BCG-PSN and thymopeptides on T-lymphocyte subsets of normal and immunosuppressed mice.
- Author:
Yunhua DENG
1
;
Yingling CHEN
;
Xingping CHEN
;
Yongxi LI
;
Liyi ZHOU
Author Information
1. Department of Dermatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
- Publication Type:Journal Article
- MeSH:
Adjuvants, Immunologic;
pharmacology;
Animals;
Female;
Immunocompromised Host;
Male;
Mice;
Mycobacterium bovis;
chemistry;
Nucleic Acids;
pharmacology;
Peptide Fragments;
pharmacology;
Polysaccharides, Bacterial;
pharmacology;
T-Lymphocyte Subsets;
drug effects;
Thymus Gland;
chemistry
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2003;23(4):339-347
- CountryChina
- Language:English
-
Abstract:
To compare the effects of polysaccharide nucleic acid fraction of bacillus calmette guerin (BCG-PSN) and thymopeptides on T-lymphocytes of normal and immunosuppressed mice, CD4+ and CD8+ T-lymphocyte subsets of single nucleic cell in thymus, spleen and peripheral blood were detected successively by flow cytometry after application of BCG-PSN and thymopeptides. Meanwhile, CD4+/CD8+ ratio was also calculated. The results showed that both BCG-PSN and thymopeptides could decrease the proportion of CD4+ CD8+ T-lymphocyte subsets in the thymus, at the same time increase CD4+ T-lymphocyte, CD8+ T-lymphocyte proportion in the three tissues. The fluctuation in amplitude was greater in thymopeptides group than that in BCG-PSN group. It is concluded that acting location of thymopeptides is in thymus, its stimulating action is stronger than that of BCG-PSN, while BCG-PSN not only accelerates the differentiation in thymus, but also has some direct stimulation to peripheral CD4+ T-lymphocytes, and can maintain CD4+/CD8+ ratio within normal range. So, BCG-PSN is safer.
