Suramin inhibits the in vitro expression of encephalitis B virus proteins NS3 and E.
- Author:
Keshu XU
1
;
Hongyu REN
;
Jianwen ZHU
;
Yun YANG
;
Fang LIAO
Author Information
1. Department of Gastroenterology, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022.
- Publication Type:Journal Article
- MeSH:
Antiviral Agents;
pharmacology;
Carcinoma, Hepatocellular;
pathology;
virology;
Cell Line, Tumor;
Encephalitis Virus, Japanese;
Humans;
Liver Neoplasms;
pathology;
virology;
RNA Helicases;
RNA, Viral;
drug effects;
Serine Endopeptidases;
Suramin;
pharmacology;
Viral Envelope Proteins;
biosynthesis;
genetics;
Viral Nonstructural Proteins;
biosynthesis;
genetics;
Virus Replication;
drug effects
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2003;23(4):375-379
- CountryChina
- Language:English
-
Abstract:
In this study, the mechanism by which Suramin inhibits the replication of epidemic encephalitis B virus was explored to provide a theoretical basis for its further application in clinical practice. After viral infection of HepG2 and IMR-32 cells, different concentrations of Suramin were added to the culture media, and then the cultural supernatants and infected cells were collected 48 h later. For the evaluation of the curative effect, cytopathic effect (CPE), virus titers, the expression of viral protein and viral RNA were determined by Western blot, RT-PCR and in vitro RNA synthesis, respectively. At the concentration of 50 microg/ml of Suramin, HepG2 and IMR-32 infected with epidemic encephalitis B virus decreased by 51.8% and 0.03% respectively, as compared with controls. It was suggested that expression of encephalitis B virus proteins NS3 and E was notably reduced by Suramin. This is especially true of E protein. At RNA level, however, no difference in RNA virus was found between Suramin-treated virus and non-treated cells. Our results suggest that Suramin can inhibit viral replication by blocking the production of viral proteins.
