Role of extracelluar regulated protein kinases in FTY720-induced apoptosis of leukemia cell lines HL-60 and U937.
- Author:
Dengju LI
1
;
Yaozhen ZHANG
;
Xiangrong HU
;
Wenjing CAO
;
Wei HUANG
Author Information
1. Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Cell Division;
drug effects;
Extracellular Signal-Regulated MAP Kinases;
metabolism;
Fingolimod Hydrochloride;
HL-60 Cells;
Humans;
Immunosuppressive Agents;
pharmacology;
Mitogen-Activated Protein Kinase 1;
metabolism;
Mitogen-Activated Protein Kinase 3;
metabolism;
Phosphorylation;
Propylene Glycols;
pharmacology;
Signal Transduction;
Sphingosine;
analogs & derivatives;
U937 Cells
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2004;24(1):45-47
- CountryChina
- Language:English
-
Abstract:
The effects of a novel immunosuppressive agent FTY720 on proliferation inhibition and apoptosis of acute leukemia cell lines HL-60 and U937, and the role of extracelluar regulated protein kinase (ERK) in the course of proliferation inhibition and apoptosis induced by FTY720 were studied. The proliferation inhibition rate of HL-60 and U937 cells by various concentrations of FTY720 was detected by MTT assay. Cell apoptosis was detected by DNA fragment analysis and flow cytometry. The phosphorylated ERK1/2 protein expression was observed by Western blotting. The change of intracellular distribution of ERK1/2 protein was identified by SP immunohistochemical staining. The results showed that FTY720 could inhibit the growth of HL-60 and U937 cells effectively in a dose-dependent manner. After incubation with FTY720 for 24 h, apoptosis was observed in HL-60 and U937 cells. The intracellular expression of phosphorylated ERK1/2 protein was also down-regulated and the distribution of ERK1/2 protein in cell nuclear was reduced during FTY720-induced apoptosis. So, that FTY720 inhibited ERK1/2 phosphorylation might mediate the role of FTY720-induced apoptosis and proliferation inhibition of leukemia cells.
