Role of VLDL receptor in the process of foam cell formation.
- Author:
Shen QU
1
;
Fan WU
;
Jun TIAN
;
Yinghong LI
;
Yan WANG
;
Yuzhe WANG
;
Yiqiang ZONG
Author Information
1. Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
- Publication Type:Journal Article
- MeSH:
Animals;
Arteriosclerosis;
metabolism;
pathology;
Cells, Cultured;
Cholesterol, LDL;
metabolism;
pharmacology;
Female;
Foam Cells;
cytology;
metabolism;
Lipoproteins, VLDL;
pharmacology;
Macrophages, Peritoneal;
cytology;
metabolism;
Mice;
Receptors, LDL;
metabolism;
Triglycerides;
metabolism
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2004;24(1):1-8
- CountryChina
- Language:English
-
Abstract:
The role of very low density lipoprotein receptor (LVLDR) in the process of foam cell formation was investigated. After the primary cultured mouse peritoneal macrophages were incubated with VLDL, beta-VLDL or low density lipoprotein (LDL), respectively for 24 h and 48 h, foam cells formation was identified by oil red O staining and cellular contents of triglyceride (TG) and total cholesterol (TC) were determined. The mRNA levels of LDLR, LDLR related protein (ILRP) and VLDLR were detected by semi-quantitative RT-PCR. The results demonstrated that VLDL, beta-VLDL and LDL could increase the contents of TG and TC in macrophages. Cells treated with VLDL or beta-VLDL showed markedly increased expression of VLDLR and decreased expression of LDLR, whereas LRP was up-regulated slightly. For identifying the effect of VLDL receptor on cellular lipid accumulation, ldl-A7-VR cells, which expresses VLDLR and trace amount of LRP without functional LDLR, was used to incubate with lipoproteins for further examination. The results elucidated that the uptake of triglyceride-rich lipoprotein mediated by VLDLR plays an important role in accumulation of lipid and the formation of foam cells.
